By John J. Bozzola (auth.), John Kuo (eds.)
The revised and increased moment version of this e-book offers the most recent expertise in electron microscopy, whereas preserving the practicality and accessibility of the acclaimed first variation. just like the first version, this quantity offers transparent, concise directions on processing organic specimens and comprises dialogue at the underlying rules of the vast majority of the procedures provided. Electron Microscopy includes significant parts of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM zone covers numerous key recommendations, together with: traditional specimen coaching tools for classy cells and biomedical and plant tissues; cryospecimen training by way of high-pressure freezing and cryoultramicrotomy destructive staining and immunogold labeling strategies; and TEM crystallography and cryo-TEM tomography. The SEM zone equally attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy strategies, in addition to the appliance of X-ray microanalysis. Protocols for the appliance of X-ray microanalysis to SEM and mass spectrometry finish the volume.
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