By Salvatore A. E. Marras (auth.), Vladimir V. Didenko MD, PhD (eds.)
Although fluorescent probes and assays, which use power move (ET) for tracking DNA reactions, have expanded lately, in the past there were no manuals summarizing the numerous varied protocols and probe designs. Fluorescent power move Nucleic Acid Probes: Designs and Protocols is the 1st booklet to supply this type of assortment. within the quantity, hands-on experts-often the unique builders of the technique-comprehensively describe a number of fluorescent probes and units that use ET, together with molecular beacons, molecular holiday lighting fixtures, TaqMan® probes, LUX™ primers, Invader® assay, aptazymes, DNAzymes, molecular machines, biosensors, and good judgment gates for molecular-scale computation. Merging paintings on nanotechnology and fluorescent probes, the authors gather the 1st accomplished therapy of all of the key DNA- and RNA-based ET probes and current ideas for his or her optimized customized layout. They talk about either fluorescence resonance strength move (FRET)-based and non-FRET-based constructs, and supply a whole set of options to observe DNA and RNA reactions, akin to hybridization, amplification, cleavage, folding, and institutions with proteins, different molecules, and steel ions. precise protocols are supplied for distance selection in protein-DNA complexes and the detection of topological DNA adjustments, mutations, and single-nucleotide polymorphisms. The protocols stick to the profitable tools in Molecular Biology™ sequence layout, each one supplying an overview of the rules in the back of the procedure, step by step precise directions, and finished troubleshooting.
Exhaustive and state of the art, Fluorescent strength move Nucleic Acid Probes: Designs and Protocols is a useful source for either amateur and skilled researchers who desire to use the newest advancements in fluorescent probes in any box of molecular biology.
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