By V. V. Didenko
The 1st complete therapy of power move (ET) nucleic acid probes. Hands-on specialists completely describe all of the significant probes, either fluorescence resonance power move (FRET)-based and non-FRET-based, and supply a whole set of ideas to observe DNA and RNA reactions, together with hybridization, amplification, cleavage, folding, and institutions with proteins, different molecules, and steel ions. optimum layout techniques for custom designed ET probes are provided, in addition to thoughts for distance choice in protein-DNA complexes and the detection of topological DNA adjustments, mutations, DNA breaks and unmarried nucleotide polymorphisms. Merging paintings on nanotechnology, and fluorescent probes, authors describe intimately the layout and alertness of ET-using molecular units, corresponding to biosensors, molecular machines, and good judgment gates for molecular scale computation.
Read or Download Fluorescent Energy Transfer Nucleic Acid Probes: Designs And Protocols (Methods in Molecular Biology Vol 335) PDF
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